學系成員

(1) Kao C-M., Chen K-F., Kuo P-S., Chien C-C., Lu C-W., Chen S-C. (2025) Mechanistic insights into chloroethene dechlorination by Dehalococcoides mccartyi strain CWV2: A multi-omics perspective. Water Research. 277: 123347.

(2) Horng YT, Chien CC, Dewi Panjaitan NS, Tseng SW, Chen HW, Yang HC, Chen YY, Soo PC. (2025) Sucrose reduces biofilm formation by Klebsiella pneumoniae through the PTS components ScrA and Crr. Biofilm. 9:100269.

(3) Tran Thi Be Lan, Yi-Chen Tsai, Zhi-Yi Huang, Yi-Lun Chen, Glemarie C. Hermosa, Kuan-Wei Lu, Chih-Ching Chien, An-Cheng Aidan Sun. (2024) Modification and Characterization of Tricalcium Silicate Bio-ceramic Powders Synthesized by Sol-Gel Process for Potential Application in Dental Treatment, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 690: 133743.

(4) Ou, J.H., Wang, C.C., Verpoort, F., Chien, C.C., Zhong, H.B., Kao, C.M. (2024). Development of innovative and green adsorbents for in situ cleanup of fluoridepolluted groundwater: Mechanisms and field-scale studies. Chemosphere, 350:141035.

(5) Wei-Han Lin; C.-C. Chien ; Jiun-Hau Ou; Ying-Liang Yu; Ssu-Ching Chen; ChihMing Kao (2023). Cleanup of Cr(VI)-polluted groundwater using immobilized bacterial consortia via bioreduction mechanisms.. Journal of Environmental Management, 339:117947.

(6) Shan-Yu Chen; Tzu-Chia Peng; Shan-Ze Huang; C.-C. Chien (2022). Isolation of an ectoine-producing Sinobaca sp. and identification of genes that are involved in ectoine biosynthesis. Fems Microbiology Letters, 369:fnac046.

(7) Horng YT, Dewi Panjaitan NS, Chang HJ, Wei YH, Chien CC, Yang HC, Chang HY, Soo PC. (2022). A protein containing the DUF1471 domain regulates biofilm formation and capsule production in Klebsiella pneumoniae. Journal of Microbiology, Immunology and Infection, 55(6 Pt 2):1246-1254.

(8) Wei-Chuan Chen, Tsang-Hao Liu, Li-Fen Wang, Chih-Ching Chien, Shan-Yu Chen, Yu-Hong Wei (2022). Enhanced production and characterization of coenzyme Q10 from Rhodobacter sphaeroides using a potential fermentation strategy. Journal of the Taiwan Institute of Chemical Engineers, 137:104201.

(9) Chen, W.T., Chien, C.C., Ho, W.S., Ou, J.H., Chen, S.C., Kao, C.M. (2022) Effects of treatment processes on AOC removal and changes of bacterial diversity in a water treatment plant. Journal of Environmental Management (J Environ Manage.) 311:114853. (June 1)

(10) Lo, K.H., Lu, C.W., Chien, C.C., Sheu, Y.T., Lin, W.H., Chen, S.C., Kao, C.M.* (2022) Cleanup chlorinated ethene-polluted groundwater using an innovative immobilized Clostridium butyricum column scheme: A pilot-scale study. Journal of Environmental Management (J Environ Manage.) 311:114836.

(11) Kao, C.-M., Chen, S.-C., Liao, Z.-Y., Wen, S.-S., Chien, C.-C. (2021) Characterization of two chromate reducing bacteria isolated from heavy metal contaminated soil. Biologia. (Dec) 76, 3909–3917.

(12) Wahyuni, E.A., Chen, C.Y., Wu, H.N., Chien, C.-C., Chen, S.-C. (2021) Propolis alleviates 4-aminobiphenyl-induced oxidative DNA damage by inhibition of CYP2E1 expression in human liver cells. Environmental Toxicology. 36(8), 1504-1513.

(13) Lin, WH, Chien, CC, Lu, CW, Hou, D, Sheu, YT, Chen, SC, Kao, CM. (2021) Growth inhibition of methanogens for the enhancement of TCE dechlorination. Science of The Total Environment 787, 15 September 2021, 147648.

(14) Panjaitan NSD, Horng YT, Chien CC, Yang HC, You RI, Soo PC. (2021) The PTS components in Klebsiella pneumoniae affect bacterial capsular polysaccharide production and macrophage phagocytosis resistance. Microorganisms. 2021 Feb 8; 9(2):335.

(15) Luo, S.G., Chien, C.C., Sheu, Y.T., Verpoort, F., Chen, S.C., Kao, C.M. (2021) Enhanced bioremediation of trichloroethene-contaminated groundwater using modified γ-PGA for continuous substrate supplement and pH control: Batch and pilot-scale studies. Journal of Cleaner Production 278,123736

(16) Lin, W.-H., Chen, S.-C., Chien, C.-C., Tsang, D.C.W., Lo, K.-H., Kao, C.-M. (2020) Application of enhanced bioreduction for hexavalent chromium-polluted groundwater cleanup: Microcosm and microbial diversity studies. Environmental Research 184,109296

(17) Fu, C.-C., Wu, C.-Y., Chien, C.-C., Hsu, T.-H., Ou, S.-F., Chen, S.-T., Wu, C.-H., Hsieh, C.-T.*, Juang, R.-S.*, Hsueh, Y.-H.* (2020) Polyethylene glycol₆₀₀₀/carbon nanodots as fluorescent bioimaging agents. Nanomaterials 10(4),677

(18) Chen, W.-C., Yuan, F.-W., Wang, L.-F., Chien, C.-C., Wei, Y.-H.* (2020) Ectoine production with indigenous Marinococcus sp. MAR2 isolated from the marine environment. Preparative Biochemistry and Biotechnology 50(1), pp. 74-81

(19) Lo, K.-H., Lu, C.-W., Lin, W.-H., Chien, C.-C., Chen, S.-C.**, Kao, C.-M.* (2020) Enhanced reductive dechlorination of trichloroethene with immobilized Clostridium butyricum in silica gel. Chemosphere 238,124596

(20) Chen, S.C., Lin, W.H., Chien, C.C., Tsang, D.C.W., Kao, C.M.* (2018) Development of a two-stage biotransformation system for mercury-contaminated soil remediation. Chemosphere, 200, 266-273.

(21) Liao, H.-Y., Chien, C.-C., Tang, P., Chen C.-C., Chen, C.-Y., Chen, S.-C.*(2018) The integrated analysis of transcriptome and proteome for exploring the biodegradation mechanism of 2, 4, 6-trinitrotoluene by Citrobacter sp. Journal of Hazardous Materials, 349, 79-90.

(22) Lin, H.-D., Hsu, L.-S., Chien, C.-C., Chen, S.-C.* (2018) Proteomic analysis of ametryn toxicity in zebrafish embryos. Environmental Toxicology, 33 (5), 579-586.

(23) Kao, C.-M., Wei, S.-F., Chen, S.C., Yao, C.-L., Ma, C., Chien, C.-C.* (2018) Biotransformation of trinitrotoluene by Citrobacter sp. YC4 and evaluation of its cyto-toxicological effects. FEMS Microbiology Letters, 365 (1), art. no. fnx256.

(24) Jeng, W.-Y., Panjaitan, N.S.D., Horng, Y.-T., Chung, W.-T., Chien, C.-C., Soo, P.-C.* (2017) The negative effects of KPN00353 on glycerol kinase and microaerobic 1,3-propanediol production in Klebsiella pneumoniae. Frontiers in Microbiology 8(DEC): 2441.

(25) Liang, S.-H., Hsu, D.-W., Lin, C.-Y., Kao, C.-M., Huang, D.-J., Chien, C.-C., Chen, S.-C., Tsai, I. J., Chen, C.-C. (2017) Enhancement of microbial 2,4,6-trinitrotoluene transformation with increased toxicity by exogenous nutrient amendment. Ecotoxicology and Environmental Safety 138:39-46.

(26) Lai, Y.C., Chien, C.C., Yang, Z.H., Surampalli, Rao Y., Kao, C.M.* (2017) Developing an Integrated Modeling Tool for River Water Quality Index Assessment. Water Environment Research 89(3):260-273.

(27) Kao, C.-M., Lin, B.-H., Chen, S.-C., Wei, S.-F., Chen, C.-C., Yao, C.-L., Chien, C.-C. (2016) Biodegradation of trinitrotoluene (TNT) by indigenous microorganisms from TNT-contaminated soil, and their application in TNT bioremediation. Bioremediation J 20(3): 165-173.

(28) Kao, C.-M., Liao, H.-Y., Chien, C.-C., Tseng, Y.-K., Tang, P., Lin, C.-E., Chen, S.-C. (2016) The change of microbial community from chlorinated solvent-contaminated groundwater after biostimulation using the metagenome analysis. J Hazard Mater 302: 144-150.

(29) Horng, Y-T., Jeng, W-Y., Chen,Y-Y., Liu, C-H., Dou, H-Y., Lee, J-J., Chang, K-C., Chien, C-C., Soo, P-C. (2015) Molecular analysis of codon 548 in the rpoB gene involved in Mycobacterium tuberculosis resistance to rifampicin. Antimicrob. Agents Chemother. (Accepted) [SCI]

(30) Chen, L.-C., Chueh, T.-C., Tuan, Y.-F., Chen, C.-C., Chien, C.-C., Lee, H.-Y., Chen, S.-C.* (2015) Activation of MAPK pathways and downstream transcription factors in 2-aminobiphenyl-induced apoptosis. Environ Toxicol (In press) [SCI]

(31) Sheu, Y.T., Chen, S.C., Chien, C.C., Chen, C.C., Kao, C.M.* (2015) Application of a long-lasting colloidal substrate with pH and hydrogen sulfide control capabilities to remediate TCE-contaminated groundwater. J Hazard Mater 284: 222-232. [SCI]

(32) Chien C-C* , Kao C-M, Chen D-Y, Chen SC, Chen C-C. (2014) Biotransformation of trinitrotoluene (TNT) by Pseudomonas spp. isolated from a TNT-contaminated environment. Environ Toxicol Chem 33 (5):1059-1063. [SCI]

(33) Chien C-C *, Wang L-J, Lin W-R (2014) Polyhydroxybutyrate accumulation by a cadmium-resistant strain of Cupriavidus taiwanensis. J Taiwan Ins Chem Eng 45 (4): 1164-1169. [SCI]

(34) Chien, C.-C.*, Lin, B.-C., Wu, C.-H. (2013) Biofilm formation and heavy metal resistance by an environmental Pseudomonas sp. Biochem Eng J 78:132-137. [SCI]

(35) Che5n, W.-C., Yu, W.-J., Chang, C.-C., Chang, J.-S., Huang, S.-H., Chang, C.-H., Chen, S.-Y., Chien, C.-C., Yao, C.-L., Chen, W-M., Wei, Y.-H.* (2013) Enhancing production of prodigiosin from Serratia marcescens C3 by statistical experimental design and porous carrier addition strategy. Biochem Eng J 78:93-100. [SCI]

(36) Horng, Y.-T., Chien, C.-C., Huang, C.-T., Wei, Y.-H., Chen, S.-Y., Lan, J.C.-W., Soo, P.-C.* (2013) Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with co-expressed propionate permease (prpP), beta-ketothiolase B (bktB), and propionate-CoA synthase (prpE) in Escherichia coli. Biochem Eng J 78:73-79. [SCI]

(37) Chien, C.-C.*, Huang, C.-H., Lin, Y.-W. (2013) Characterization of a heavy metal translocating P-type ATPase gene from an environmental heavy metal resistance Enterobacter sp. isolate. Appl Biochem Biotechnol 169(6): 1837-1846. [SCI]

(38) Chien, C-C.*, Li, H-H., Soo, P-C., Chen, S-Y., Wei, Y-H., Chen, W-M. (2012) Effects of different substrate composition on biosynthesis of polyhydroxybutyrate-co-hydroxyvalerate by recombinant Escherichia coli. Appl Biochem Biotechnol 166(3): 796-804. [SCI]

(39) Chien, C-C.*, Jiang, M-H., Tsai, M-R., Chien, C-C. (2011) Isolation and characterization of an environmental cadmium and tellurite resistant Pseudomonas strain. Environ Toxicol Chem 30(10): 2202-2207. [SCI].

(40) Chu, F.-Y.*, Su, F.-H., Cheng, S.-H., Lin, Y.-S., Li, C.-Y., Chien, C.-C., Lin, Y.-C., Chiang, S.-Y. (2011) Hepatitis B surface antigen confirmatory testing for diagnosis of hepatitis b virus infection in . J Med Virol 83:1514–1521. [SCI]

(41) Horng, Y-T., Chien, C-C., Wei, Y-H., Chen, S-Y., Lan, J.C-W., Sun, Y-M., Soo, P-C.* (2011) Functional cis-expression of phaCAB genes for poly(3-hydroxybutyrate) production by Escherichia coli. Lett Appl Microbiol 52(5):475-483. [SCI]

(42) Chen S.C., Chen C. S., Zhan K-V., Yang K-H., Chien C-C., Shieh B-S., Chen W-M. (2011) Biodegradation of methyl tert-butyl ether (MTBE) by Enterobacter sp. NKNU02. J Hazard Mater 186 (2-3): 1744-1750 [SCI]

(43) Chien, C-C.*, Hong, C-C., Soo, P-C., Wei, Y-H., Chen, S-Y., Cheng, M-L., Sun, Y-M. (2010) Functional expression of phaCAB genes from Cupriavidus taiwanensis strain 184 in Escherichia coli for polyhydroxybutyrate production. Appl Biochem Biotechnol 162(8):2355–2364 [SCI]

(44) Horng, Y-T., Chang, K-C., Chou, T-C., Yu, C-J., Chien, C-C., Wei, Y-H., Soo, P-C.* (2010) Inactivation of dhaD and dhaK abolishes by-product accumulation during 1,3-propanediol production in Klebsiella pneumoniae. J Ind Microbiol Biotechnol 37(7):707–716. [SCI]

(45) Kao, C-M., Chen, CS., Tsa, F-Y., Yang, K-H., Chien, C-C., Liang, S-H., Yang, C-A., Chen, SC.* (2010) Application of real-time PCR, DGGE fingerprinting, and culture-based method to evaluate the effectiveness of intrinsic bioremediation on the control of petroleum-hydrocarbon plume. J Hazard Mater 178(1-3):409-416. [SCI]

(46) Horng, Y-T., Chang, K-C., Chien, C-C., Wei, Y-H., Sun, Y-M., Soo, P-C.* (2010) Enhanced polyhydroxybutyrate (PHB) production via the coexpressed phaCAB and vgb genes controlled by arabinose PBAD promoter in Escherichia coli. Lett Appl Microbiol 50(2): 158-167. [SCI]

(47) Hung, HF., Kuo, YM.*, Chien, CC., Chen, CC. (2010) Use of floating balls for reducing bacterial aerosol emissions from aeration in wastewater treatment processes. J Hazard Mater 175(1-3):866-871. [SCI]

(48) Chien, C-C.*, Han, C-T. (2009) Tellurite resistance and reduction by a Paenibacillus sp. isolated from heavy metal–contaminated sediment. Environ Toxicol Chem 28(8):1627-1632. [SCI]

(49) Chien, C-C.*, Ho, L-Y. (2008) Polyhydroxyalkanoates production from carbohydrates by a genetic recombinant Aeromonas sp. Lett Appl Microbiol 47:587-593. [SCI]

(50) Chien, C-C.*, Tsai, M-L., Chen, C-C., Chang, S-J., Tseng, C-H. (2008) Effects on tyrosinase activity by the extracts of Ganoderma lucidum and related mushrooms. Mycopathologia 166(2):117-120. [SCI]

(51) Wei, Y-H.*, Cheng, C-L., Chien C-C., Wan, H-M. (2008) Enhanced di-rhamnolipid production with an indigenous isolate Pseudomonas aeruginosa J16. Process Biochem 43(7): 769-774. [SCI]

(52) Chien, C-C.* (2008) Taurine serves as sole source of nitrogen for aerobic and anaerobic growth by Klebsiella sp. World J Microbiol Biotechnol 24(6): 895-898. [SCI]

(53) Chien, C-C.*, Kuo, Y-M., Chen, C-C., Hung, C-W., Yeh, C-W., Yeh, W-J. (2008) Microbial diversity of soil bacteria in agricultural field contaminated with heavy metals. J Environ Sci 20(3): 359-363. [SCI]

(54) Su, F-H., Cheng, S-H., Li, C-Y., Chen, J-D., Hsiao, C-Y., Chien, C-C., Yang, Y- C., Hung, H-H., Chu, F-Y. * (2007) Hepatitis B seroprevalence and anamnestic response amongst Taiwanese young adults with full vaccination in infancy, twenty years subsequent to national hepatitis B vaccination. Vaccine 25(47): 8085-8090. [SCI]

(55) Chien, C-C.*, Chen C-C, Choi, M-H., Kung, S-S., Wei, Y-H. (2007) Production of poly-ß-hydroxybutyrate (PHB) by Vibrio spp. isolated from marine environment. J Biotechnol 132(3):259-263. [SCI]

(56) Kung, S-S., Chuang, Y-C., Chen, C-H., Chien, C-C*. (2007) Isolation of polyhydroxyalkanoates (PHAs) producing bacteria using a combination of phenotypic and genotypic approach. Lett Appl Microbiol 44:364-371. [SCI]

(57) Chien, C-C.*, Hung, C-W., Han, C-T. (2007) Removal of cadmium ions during stationary growth phase by an extremely cadmium-resistant strain of Stenotrophomonas sp. Environ Toxicol Chem 26(4):664-668. [SCI]

(58) Chien, C-C.* (2005) Arylsulfonates as sole source of sulfur for Clostridium pasteurianum DSM 12136. J Basic Microbiol 45(4): 274-278. [SCI]

專書論著

(1) Chien, C-C. (2008) Polyhydroxyalkanoates: In nature, in the laboratory and in industry. In: "Biotechnology: Research, Technology and Applications."  (Editor: Felix W. Richter) Nova Science Publishers, Inc. Hauppauge, NY, . pp. 47-70.

(2) Chien, C-C.(2013) Heavy Metal Niches: Microbes and Their Metabolic Potentials. In: “Applications of Microbial Engineering.” (Editors: Vijai Kumar Gupta, Monika Schmoll, Marcio Antonio Mazutti, Minna Maki, Maria G. Tuohy) CRC Press, Taylor & Francis Group, Boca Raton, FL, USA. (ISBN 978-1-4665-8577-5) pp. 342-366.

專利

(1) 簡志青，蘇伯琦，洪育芝，陳姍玗。「一種使大腸桿菌大量表現丙酸代謝相關基因與合成聚羥基丁酯-羥基戊酯之方法」。中華民國專利申請號：100135702。

(2) 簡志青，蘇伯琦，洪育芝，陳姍玗。「藉由重組大腸桿菌以生產聚羥基丁酸酯(PHB)的方法」。中華民國專利申請號：100103238。

(3) 簡志青，孫一明，康世旭，陳昌傑，何玲儀。「可使微生物利用不同碳源以合成PHA之方法」。中華民國專利I374190，公告日:  101年10月11日。

(4) 康世旭 (Kang, S-H.)，孫一明 (Sun, Y-M.)，簡志青 (Chien, C-C.)，唐世珍 (Tang, S-C.)，陳昌傑 (Chen, C-C.)。Method for production of high purity polyhydroxyalkanoates (PHAs)。US Patent 8053219 B2 (Nov 8, 2011)。