學系成員

黃麗芬 副教授兼工院英語學士學位學程主任

學歷

University of Florida Plant Molecular and Cellular Biology 博士

臺灣大學 植物學系暨研究所 碩士

臺灣大學 植物學系暨研究所 學士

經歷

元智大學工程學院英語學士學位學程主任

元智大學 生物科技與工程研究所副教授

元智大學 生物科技與工程研究所助理教授

中研院 分生所 中研院院內博士後研究

中研院 分生所 國科會 博士後研究

University of Florida Horticultural Sciences Research Biologist

University of Florida Horticultural Sciences RA

中研院 分生所 分生所編制內研究助理

研究專長

1.植物生物科技:水稻重組蛋白表現系統(醫藥用蛋白)

2.藥用植物組織培養:天山雪蓮幹細胞培養

3.核酸偵測器研發


期刊論文

Publication:

1. Nguyen, T.M., Lu, C.A., Huang, L.F.* (2022) Applications of CRISPR/Cas9 in a rice protein expression system via an intron-targeted insertion approach. Plant Science, 315:111132. https://doi.org/10.1016/j.plantsci.2021.111132 (SCI, Impact Factor: 4.729).

2. Wu, C.L., Lin, L.F., Hsu, H.C., Huang, L.F., Hsiao, C.D., Chou, M.L. (2021) Saussurea involucrata (Snow Lotus) ICE1 and ICE2 orthologues involved in regulating cold stress tolerance in transgenic Arabidopsis. Int. J. Mol. Sci., 22, 10850. https://doi.org/10.3390/ijms221910850 (SCI, Impact Factor: 5.924).

3. Sinaga, D.S., Ho, S.L, Lu. C.A., Yu, S.M., Huang, L.F.* (2021) Knockdown expression of a MYB-related transcription factor gene, OsMYBS2, enhances production of recombinant proteins in rice suspension cells. Plant Methods, 25(17):99. doi: 10.1186/s13007-021-00799-2 (SCI, Impact Factor: 4.993). 

4. Huang, L.F.*, Sinaga, D.S., Tan, C.C., Hsieh, S.J.M., Huang, C.H. (2021) Expression of recombinant human octamer-binding transcription factor 4 in rice suspension cells. Int. J. Mol. Sci., 22: 1409. https://doi.org/10.3390/ijms22031409 (SCI, Impact Factor: 5.924).

5. Chuang, J.N., Diao, P.Y., Huang, W.S., Huang, L.F., Senapati, S., Chang, H.C. and Sun, Y.M.* (2020) Novel homogeneous anion exchange membranes for reproducible and sensitive nucleic acid detection via current–voltage characteristic measurement, ACS Applied Materials and Interfaces, 49: 54459–54472 (SCI, Impact Factor: 9.229).

6. Huang, L.F.*, Liu, Y.K., Su, S.-C., Lai, C.-C., Wu, C.-R., Chao, T.-J. Yang, Y.H. (2020) Genetic engineering of transitory starch accumulation by knockdown of OsSEX4 in rice plants for enhanced bioethanol production, Biotechnol Bioeng, 117: 933-944 (SCI, Impact Factor: 4.53).

7. Liu, Y.K., Lu, C.W., Chang, J.Y., Lu, C.F., Tan, C.C. and Huang, L.F.* (2018) Optimization of the culture medium for recombinant protein production under the control of the αAmy3 promoter in a rice suspension-cultured cell expression system, Plant Cell, Tissue and Organ Culture, 132: 383–391. (SCI, Impact Factor: 2.711).

8. Huang, L.F., Lin, K.H., He, S.L., Chen, J.L., Jiang, J.Z., Chen, B.H., Hou, Y.S., Chen, R.S., Hong, C.Y. and Ho, S.H.* (2016) Multiple patterns of regulation and overexpression of a ribonuclease-like pathogenesis-related protein gene, OsPR10a, conferring disease resistance in rice and Arabidopsis. PLoS ONE, 11(6): e0156414. doi:10.1371/journal.pone.0156414. (SCI, Impact Factor: 3.240).

9. Huang, C.K., Shen, Y.L., Huang, L.F., Wu, S.J., Yeh, C.H., Lu, C.A.* (2016) The DEAD-Box RNA Helicase AtRH7/PRH75 Participates in Pre-rRNA Processing, Plant Development and Cold Tolerance in Arabidopsis. Plant Cell Physiol., 57(1):174-91. doi: 10.1093/pcp/pcv188. (SCI, Impact Factor: 4.927).

10. Huang, L.F.*, Tan, C.C., Yeh, J.F., Liu, H.Y., Liu, Y.K., Ho, S.L., Lu, C.A. (2015) Efficient secretion of recombinant proteins from rice suspension cultured cells modulated by the choice of signal Peptide. PLoS ONE, 10(10): e0140812. doi:10.1371/journal.pone.0140812 (SCI, Impact Factor: 3.240).

11. Huang, L.F.*; Lin, J.Y.; Pan, K.Y.; Huang, C.K.; Chu, Y.K. (2015) Overexpressing Ferredoxins in Chlamydomonas reinhardtii increase starch and oil yields and enhance electric power production in a photo microbial fuel cell. Int. J. Mol. Sci., 16: 19308-19325, Link: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581298/ (SCI, Impact Factor: 5.924).

12. Huang, C.K., Lo, P.C., Huang, L.F., Wu, S.J., Yeh, C.H., Lu, C.A.* (2015) A single-repeat MYB transcription repressor, MYBH, participates in regulation of leaf senescence in Arabidopsis. Plant Mol Biol, 88(3):269-86. doi: 10.1007/s11103-015-0321-2. (SCI, Impact Factor: 4.076).

13. Liu, Y.K., Li, Y.T., Lu, C.F., Huang, L.F.* (2015) Enhancement of recombinant human serum albumin in transgenic rice cell culture system by cultivation strategy. New Biotechnology, 32(3): 328-334 (SCI, Impact Factor: 5.079).

14. Lin. Y.H., Huang. L.F., Hase, T., Huang, H. E., Feng, T.Y. (2015) Expression of plant ferredoxin-like protein (PFLP) enhances tolerance to heat stress in Arabidopsis thaliana. New Biotechnolgy, 32(2): 235-242 (SCI, Impact Factor: 5.079).

15. Chou, W.L., Huang, L.F., Fang, J.C., Yeh, C.H., Hong, C.Y., Wu, S.J., Lu, C.A. (2014) Divergence of the expression and subcellular localization of CCR4‑associated factor 1 (CAF1) deadenylase proteins in Oryza sativa. Plant Mol Biol, 85(4-5): 443-458 (SCI, Impact Factor: 4.076).

16. Chen, W.C., Lai, Y.A., Lin, Y.C., Ma, J.W., Huang, L.F., Yang, N.S., Ho, C.T., Kuo, S.C., Way, T.D. (2013) Curcumin suppresses Doxorubicin-induced epithelial-mesenchymal transition via the inhibition of TGF-β and PI3K/AKT signaling pathways in triple-negative breast cancer cells. Journal of Agricultural and Food Chemistry, 61(48): 11817~11824 (SCI, Impact factor 5.279).

17. Chen. C.C., Chen, Y.; Hsi, Y.T.; Chang, C.S.; Huang, L.F., Ho, C.T., Way T.D., Kao, J.Y. (2013) Chemical constituents and anticancer activity of Curcuma zedoaria Roscoe essential oil against non-small cell lung carcinoma cells in vitro and in vivo. Journal of Agricultural and Food Chemistry, 61(47): 11418~11427 (SCI, Impact factor 5.279).

18. Lin, Y.H., Pan, K.Y., Hung, C.H., Huang, H.E., Chen, C.L., Feng, T.Y., Huang, L.F.* (2013) Over-expression of Ferredoxin, PETF, enhances tolerance to heat stress in Chlamydomonas reinhardtii. Int. J. Mol. Sci.,14(10) 20913-20929. Link: http://www.mdpi.com/1422-0067/14/10/20913 (SCI, Impact factor: 5.924).

19. Kuo, Y.C., Tan, C.C., Ku, J.T., Hsu, W.C., Su, S.C., Lu, C.A., Huang, L.F.* (2013) Improving Pharmaceutical Protein Production in Oryza sativa. Int. J. Mol. Sci., 14: 8719-8739. Link: http://www.mdpi.com/1422-0067/14/5/8719 (SCI, Impact factor: 5.924).

20. Ho, S.L., Huang, L.F., Lu, C.A., Wang, C.C., Chen, J and Yu, S.M. (2013) Sugar starvation- and GA-inducible calcium-dependent protein kinase 1 feedback regulates GA biosynthesis and activates a 14-3-3 protein to confer drought tolerance in rice seedlings. Plant Mol Biol, 81:347-361 (equal contribution as the first author, SCI, Impact Factor: 4.076).

21. Liu, Y.K., Huang, L.F., Ho, S.L., Liao, C.Y., Liu, H.Y., Lai, Y.H., Yu, S.M. and Lu, C.A. (2012) Production of mouse granulocyte-macrophage colony stimulating factor by Gateway technology and transgenic rice cell culture. Biotechnology & Bioengineering, 109:1239-1247 (SCI, Impact Factor: 4.53).

22. Lo, Y.C., Huang, L.F., Cheng, C.L., Chen, J. and Chang, J.S. (2011) Using a starch-rich mutant of Arabidopsis thaliana as feedstock for fermentative hydrogen production. Bioresource Technology, 102:8543-8546. (SCI, Impact factor: 9.642).

23. Huang, C.K., Huang, L.F., Huang, J.J., Wu, S.J., Yeh, C.H., Lu, C.A. (2010) A DEAD-box protein, AtRH36, is essential for female gametophyte development and is involved in rRNA biogenesis in Arabidopsis. Plant and Cell Physiology, 51:694-706 (SCI, Impact factor: 4.927).

24. Lin, H.H., Huang, L.F., Su, H.C., Jeng. S.T. (2009) Effects of the multiple polyadenylation signal AAUAAA on mRNA 3'-end formation and gene expression. Planta, 230:699-712 (equal contribution as the first author, SCI, Impact Factor: 4.116).

25. Lu, C.A., Lin, C.C., Lee, K.W., Chen, J.L., Huang, L.F., Ho, S.L., Liu, H.J., Hsing, Y.I. and Yu, S.M. (2007) The SnRK1A protein kinase plays a key role in sugar signaling during germination and seedling growth of Rice. Plant Cell, 19: 2484-2499 (SCI, Impact Factor: 11.277).

26. Huang, L.F., Bocock, P.N., Davis , J.M. and Koch, K.E. (2007) Invertase regulation: a “suite” of transcriptional and post-transcriptional mechanisms. Functional Plant Biology, 34:499-507 (SCI, Impact Factor: 3.101).

27. Kohorn, B.D., Kobayashi, M., Johansen, S., Riese, J., Huang, L.F., Koch, K., Fu, S., Dotson, A. and Byers, N. (2006) An Arabidopsis cell wall-associated kinase required for invertase activity and cell growth. Plant Journal, 46:307-316 (SCI, Impact Factor: 6.486).

28. Huang, L.F., Liu, Y.K., Lu, C.A., Hsieh, S.L. and Yu, S.M. (2005) Production of human serum albumin by sugar starvation induced promoter and rice cell culture. Transgenic Research, 14:569-581 (SCI, Impact Factor: 2.788).

29. Chiang, C.M., Yeh, F.S., Huang, L.F., Tseng, T.H., Chung M.C., Wang C.S., Lur, H.S., Shaw, J.F. and Yu, S.M. (2005) Expression of a bi-functional and thermostable amylopullulanase in transgenic rice seeds leads to autohydrolysis and altered composition of starch. Molecular Breeding, 15:125-143 (SCI, Impact Factor: 2.589).

30. Chen, M.H., Huang, L.F., Li, H.M., Chen, Y.R, Yu, S.M. (2004) Signal peptide-dependent targeting of a rice α-amylase and cargo proteins to plastids and extracellular compartments of plant cells. Plant Physiology, 135: 1367-1377 (equal contribution as the first author, SCI, Impact Factor: 8.34).

* indicates corresponding author

 註: Impact Factor引用來源:Journal Citation Reports™ 2020


研究計畫

歷年科技部獎助之計畫:

執行期間    個人型計畫題目

2021-2022    應用CRISPR-Cas9系統編輯水稻基因以優化重組蛋白表現量

2017-2021    水稻細胞表現人類幹細胞轉錄因子Oct4重組蛋白的研發與應用

2014-2017    OsSEX4在水稻葉片澱粉降解角色的研究與應用

2013-2014    植物特有蛋白質醣基化基因在水稻的探討與應用

2010-2011    以甘藷作為植物分子農場大量生產顆粒細胞-巨噬細胞集落刺激因 子(GM-CSF)

2009-2010    改造水稻細胞醣化作用系統以生產高價值重組蛋白

2008-2009    14-3-3蛋白與植物葉片澱粉代謝相關酵素結合之功能分析

相關著作與專利

Patent

1. US 10,041,080 B2 MODIFIED PROMOTER SEQUENCE AND APPLICATION THEREO (2018/8/7)

2. ROC patent, No. 530503, [修飾的啟動子序列及其應用],專利權人:元智大學;發明人:黃麗芬、陳鵬文、余淑美

所有權人:元智大學;發明人:黃麗芬、陳鵬文、余淑美 (2016/04/21)

得獎事蹟

-2021/11/18於亞東醫院14樓國際會議廳參與「110 年度亞東元智智慧生技醫療創客平台成果發表會」,與曾一修醫師共同演講發表【天山雪蓮懸浮細胞萃取液對HK-2細胞上草酸鈣結晶引起的TNF-α與黏附蛋白的影響】,並獲最佳海報獎。

-2019/5/31指導助理辛娜嘉參加中央大學主辦之【2019年第十二屆桃園四校生命科技與工程研討會】壁報比賽,獲得[生技工程組]特優(第一名)。

-2018年12月帶領跨校團隊,包含元智、中央、慈濟和長庚學生助理共8人,獲教育部「2018生醫產業與新農業創新創業競賽」,醫藥B組金牌第一。

-2018年6月指導外籍學生古瑪莉,參加2018第11屆桃園四校生命科學領域聯合學術研討會海報競賽,獲生化生醫組第三名甲等。

-2017年9月於韓國POSTECH生技中心參與第一屆植物製藥物亞洲會議,獲得最佳海報獎 (Best Poster Award)。 

-2016年指導張姓研究生參加中央大學主辦之【2016第九屆桃園四校生命科技領域聯合學術研討會】壁報比賽,獲得[生技工程組]特優(第一名)。

-104學年度榮獲元智大學[青年學者研究獎] 

-2015年指導褚姓研究生參加中原大學主辦之【2015第八屆桃園四校生命科技領域聯合學術研討會】壁報比賽,獲得[生化生醫工程組]優選(第二名)。

-2014年指導褚姓研究生參加 10th International Symposium on Biocatalysis and Agricultural Biotechnology (ISBAB),榮獲"Best poster Award" 。 

-2014年指導劉姓研究生榮獲"元智大學生技所第六屆學生學術研討會論文競賽"二年級第一名。 

-2014年指導劉姓研究生榮獲"2014第19屆生物技術暨生物工程研討會"壁報論文競賽-優勝。 

-2014年指導劉姓研究生參加長庚大學主辦之【2014第七屆桃園四校生命科技領域聯合學術研討會】壁報比賽,獲得[生化生醫工程組]優選。

-2013年指導林姓研究生榮獲"2013第18屆生物技術暨生物工程研討會"壁報論文競賽-優勝。 

-2013年指導外籍研究生丹寧榮獲"2013第18屆生物技術暨生物工程研討會"英語口頭論文競賽-佳作。 

-2013年指導郭姓研究生榮獲"2013第18屆生物技術暨生物工程研討會"英語口頭論文競賽-第一名。 

-2013年指導蘇姓研究生榮獲"第六屆桃園四校生命科技領域聯合學術研討會"論文海報競賽-生技工程組第一名。 

-2013年指導外籍研究生丹寧榮獲"第六屆桃園四校生命科技領域聯合學術研討會"論文海報競賽-生技工程組第三名。 

-2012年指導譚姓學生榮獲"2012中華植物學會年會"壁報論文競賽優等暨最佳人氣海報。  

-2012年指導吳姓研究生榮獲"第十七屆生化工程研討會"壁報論文發表競賽優勝 。 

-2012年指導林姓研究生榮獲"第十七屆生化工程研討會"壁報論文發表競賽優勝。  

-2012年指導譚姓研究生同學榮獲"第五屆桃園四校生命科技領域聯合學術研討會"論文海報競賽-生技工程組特優(第一名)。 

-2012年指導吳姓研究生同學榮獲"第五屆桃園四校生命科技領域聯合學術研討會"論文海報競賽-生技工程組優選(第二名)。 

-2012年榮獲執行『元智大學與亞東紀念醫院學術合作先期研發專題計畫』。 

-2011年獲邀參與Gorden Research Conference- Plant metabolic Engineering (Poster Presenter)。 

-2010年指導研究生顧同學榮獲 The 16th Symposium of Young Asian Biochemical Engineers' Community 最佳壁報獎(Genetic, protein and metabolic engineering )。 

-2009年榮獲中央研究院獎勵國內學人來院短期訪問研究。 

-2007年榮獲"第二十五屆中華植物學會暨植物科學與生質能源前瞻學術研討會"優良壁報論文獎。 

-Travel Grant Award from graduate student council of University of Florida, 2006, Gainesville, Florida, U.S.A. (May 2006) 

-Graduate School Travel Grant Award in Plant Biology 2005, American Society of Plant Biologists, Seattle, Washington, U.S.A. (July 2005) (全美植物生理學最大會議)

-Best-Presentation Award at state-wide workshop for Plant Molecular & Cellular Biology, Daytona, Florida, U.S.A. (May 2005)